Specializing in Biomolecular Testing Kits for Toxicity, Mutagenicity, and Genotoxicity.
EBPI is a biotechnology-based company specializing in the manufacture and custom application of simple, rapid, and cost-effective methods for detection and monitoring of toxic, mutagenic and genotoxic materials. In addition to being used for evaluation of existing and new chemicals, the kits have found application in assessing environmental pollution risks in drinking water, wastewater, soil and air. We are a research and development-focused laboratory and manufacturing facility continually developing new testing procedures and easy-to-use testing kits for use by research laboratories around the world.
Specializing in Biomolecular Testing Kits for Toxicity, Mutagenicity, and Genotoxicity.
EBPI is a biotechnology-based company specializing in the manufacture and custom application of simple, rapid, and cost-effective methods for detection and monitoring of toxic, mutagenic and genotoxic materials. In addition to being used for evaluation of existing and new chemicals, the kits have found application in assessing environmental pollution risks in drinking water, wastewater, soil and air. We are a research and development-focused laboratory and manufacturing facility continually developing new testing procedures and easy-to-use testing kits for use by research laboratories around the world.
Ames Test Kits.jpg "Ames Reverse Mutation Kit") 
A microplate version of the traditional "pour plate" Ames Test.
The Muta-ChromoPlateTM Kit is sensitive and user friendly. It is commonly used to detect mutagenic potential of individual chemicals or mixtures of chemicals in water, wastewater, sediment, air aqueous extracts, new chemicals, foods, cosmetics, pharmaceuticals and any other material that can be put into aqueous solution or micro-suspension, such that it can be taken up by the test strain.
The Ames ISO Basic Kit is a fluctuation assay verison of
the well-known “Ames Test” which uses a 48-well sample exposure format
on a 384-well microplate to reduce reagent and plastic used. The Ames
ISO procedure was developed to test mutagenic materials in a variety of
sources like environmental air and water samples, foods, personal care
products, and industrial effluents by the fluctuation assay which is a
variation of the traditional “Ames Test”.
The Ames ISO method provides researchers with a reliable
test method which is facile to set up and run, amenable to samples in
aqueous solution or suspension and produces an easily-detectable
colorimetric endpoint after 2-3 days of incubation at 37 °C.
This Ames test method is a modification of the Ames ISO
fluctuation assay. Bacterial strains are pre-exposed to differing
concentrations of the mutagenic compounds with a growth media in
suspension for 100 minutes. Each dilution is run in triplicate.
The samples are then transferred, mixed with a reversion
solution and plated onto a 96-well plate while using a 48-well
evaluation format. The samples are incubated for 2-3 days and the number
of revertant colonies (individual wells) are counted by colorimetric
analysis.
Genotoxicity Test Kits
A microplate genotoxicity bioassay based on the primary response of a genetically engineered bacteria to determine DNA genotoxicity damage. The SOS-ChromoTestTM Kit can be used to evaluate the presence of genotoxic agents in water, wastewater, sediment, air, chemicals, food, cosmetics, and for any material that can be put into aqueous solution or micro-suspension such that it can be taken up by the test strain.
.jpg "umu-c genotoxicity test")
A microplate toxicity bioassay, EBPI has developed the UMU-CHROMOTESTTM into a simple procedure based upon the International Organization for Standardization protocol ISO 13829 (Water Quality- Determination of the genotoxicity of water and waste water using the umu-test), which can be performed easily in a non‑specialized laboratory.
Toxicity Test Kits
EBPI is currently carrying several preparations of high quality Aliivibrio fischeri test reagents to conduct rapid toxicity assessments in a wide range of environmental samples.
The BioTox™ line of reagents and kits are designed to function independently and include all necessary components.
The reagents are standardized for use with ISO 11348-3: Water quality – Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test). ISO 21338:2010 Water quality — Kinetic determination of the inhibitory effects of sediment, other solids and coloured samples on the light emission of Vibrio fischeri (kinetic luminescent bacteria test)
.jpg "Toxicity Testing")  A microplate toxicity bioassay to determine acute or chronic toxicity in water, effluents, and other liquids.
The Toxi-ChromoTestTM Kits endpoint is either partial or complete enzyme inhibition related to the toxic stress the test organism is exposed to. It’s endpoint is a simple color change that can be determined visually or using a plate reader at a wavelength of 610nm.
Toxicity Tests with Ciliate Protozoans TK11 - Chronic PROTOXKIT F with Tetrahymena thermophila (freshwater tests - 6 tests per kit)
Toxicity Tests with RotifersTK21 - Acute ROTOKIT F with Brachionus calyciflorus (freshwater tests - 6 tests per kit)rotifer RotoxkitF ToxicityTest  TK22 - Acute ROTOKIT M with Brachionus plicatilits (estuarine/marine test - 6 tests per kit) TK23 - Short-chronic ROTOXKIT F Brachionus calyciflorus (freshwater - 3 tests per kit)
Toxicity Tests with CrustaceansTK31 - THAMNOTOXKIT F with Thamnocephalus platyurus (freshwater tests - 6 tests per kit) TK32 - ARTOXKIT M with Artemia franciscana (estuarine/marine tests - 6 tests per kit) TK33 - DAPHTOXKIT F magna with Daphnia magna (freshwater - 6 tests per kit) TK35 - Acute CERIODAPHTOXKIT F with Ceriodaphnia dubia (freshwater - 6 tests per kit)daphnia ToxicityTest TK36 - OSTRACODTOXKIT F with Heterocypris incongruens (freshwater sediment 3-5 tests per kit) TK37 - RAPIDTOXKIT with Thamnocephalus platyurus (freshwater tests - up to 45 tests per kit)
Toxicity Tests with Micro-Algae TK41 - ALGALTOXKIT F with Selenastrum capricornutum (freshwater tests - 2 tests per kit) TK42 - Marine ALGALTOXKIT with Phaeodactylum tricornutum (estuarine/marine tests - 2 tests per kit)
Toxicity Tests with Higher PlantsTK61 - PHYTOTOXKIT with seeds of 3 higher plants (1 monocotyl & 2 dicotyls) TK62 - PHYTOTESTKIT with seeds of 3 higher plates (1 monocotyl & 2 dicotyls)
TK63 - SPIRODELA DUCKWEED TOXKIT with Spirodela polyrhiza (2 tests per kit)
A convenient test for quantitative measure of total coliforms and E. Coli bacteria. The ColiPlate test is designed to meet regulatory guidelines and quantifies density of target bacteria, coliforms and E. Coli, ranging from 5 to 5,000 colony forming-units (cfu) per 100 mL sample, without dilutions.
 APPLICATION OF TOXKIT MICROBIOTESTS FOR TOXICITY EVALUATION OF THE OIL SPILL IN THE GULF OF MEXICO
Shortly after the Deepwater Horizon's oil pipe break in the Gulf ofMexico,decision by government authorities along with management groups to use the marine microbiotest with Brachionus plicatilis (Rotoxkit M) for detection and quantification of the toxic hazard of the waters in the Gulf of Mexico was put into place.
The Rotoxkit M culture/maintenance free assay was selected for this application in part because of its portability and ease of use and its ability to be rapidly deployed for use on research vessels as was required in the Gulf of Mexico MicroBioTests Inc have developed these kits with ISO, EPA, and ASPM standards in mind. The full Toxkit line of products can be found under the MicroBioTests link on either main menu.
A complete list of components of these kits can be found under the "MicroBioTest - Component" link on the key concepts menu. Please contact EBPI for further information regarding any of the toxicity testing kit manufactured or distributed by EBPI. |
.jpg "SOS Gene Response DNA Damage")
