The Toxi-ChromoTest kit is designed for Rapid Detection of Acute or Chronic Toxicity.
The procedure exposes the bacteria to the toxicants in the sample for a short (90-minute) incubation period. After the incubation period, a chromogenic substrate is added. If the sample is toxic, no colour will develop and if the sample is non-toxic, a distinctive blue colour quickly develops. The kit provides a clear, completely objective measurement of the toxicity of the sample by a simple visual qualitative evaluation of the colour obtained, or quantitatively by spectrophotometry using a micro-plate reader. Te
The Toxi-ChromoTest kit contains three sets of components and, therefore, allows four separate testing procedures to be completed at the same or different times.
Each Toxi-ChromoTest kit can be used for a total of 192 tests. These tests must include standards and controls (using at least 32 units.) Thus, a single kit can screen 160 samples for yes/no response without replicates.
It is necessary to allow sample dilutions and replicates, thus testing of no more than 20 samples with each kit for reliable and quantitative bioassays is recommended.
Applications for the Toxi-ChromoTest include screening of effluents for changes in toxicity levels, monitoring of municipal discharges for occasional spills, inclusion in a "battery of tests" for monitoring aqueous environmental samples, detection of potential toxicity in raw materials, and use as a convenient research and teaching tool.
Used within Universities across North America, the Toxi-ChromoTest kit can be tailored to meet the demands of any university student. Used in support of a graduate thesis, or as a learning tool for laboratory use at the undergraduate level.
In 2006, the Toxi-ChromoTest was verified by the ETV program under the U.S. Environmental Protection Agency.
The Toxi -ChromoTest offers the following advantages over traditional methods:
Testing of pharmaceuticals for toxic activity.
Testing of industrial effluents for presence of possible toxic compounds.
Screening of municipal discharges for possible routine presence or spills of toxic compounds.
Screening of surface and ground water for toxic residues.
Screening of potable water supplies for the presence of chemicals with toxic potential.
Screening of water soluble air pollutants for toxic agents.
Evaluation of pure or complexed raw mixtures for potential toxicity.
A convenient and easy to use teaching look for university and college laboratories.
The Toxi-ChromoTest kit is designed to be user friendly, reliable, sensitive, rapid, low cost and contains all plastics needed to complete the assay.